Peroxidase

BBI Enzymes is the world’s leading producer of Horseradish Peroxidase (HRP) and has supplied the diagnostics industry with this key raw material for in excess of 40 years.

HRP is used in a wide range of liquid clinical chemistry reagents, dry powder reagents and test strips. It is also commonly used as a label in immunoassays such as ELISA, and in blotting and histochemistry.  Once conjugated to antibodies, antigens, or streptavidin, its rapid turnover rate gives high sensitivity over short incubation times. Peroxidase conjugates may give higher backgrounds than other enzyme conjugates, hence extra blocking steps, e.g. the introduction of APO-Peroxidase, may be necessary. It can be used with a variety of substrates producing precipitated or soluble chromogens, or with chemiluminescent and fluorogenic substrates for enhanced sensitivity.

We have developed close relationships with horseradish growers in both the Northern and Southern hemispheres giving an unrivalled year-round supply of specially selected and cultivated roots.  Our South Africa facility routinely processes two tonnes of roots per day allowing the consistent production of multi-kg batches of clinical chemistry grade and multi-100g batches of immunoassay grade HRP using our optimised purification procedures.

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